Journal: Applied and Environmental Microbiology
Article Title: Characterization of Shigella virulence factor intracellular spread A (IcsA, or VirG) functional epitopes against S. flexneri 2a and S. sonnei invasion and adherence
doi: 10.1128/aem.01175-25
Figure Lengend Snippet: S. sonnei and S. flexneri 2a bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.
Article Snippet: Briefly, S. sonnei or S. flexneri 2a Congo red strain bacteria (~ 2 × 10 7 CFUs in 30 μL PBS) were mixed with 30 μL heat-inactivated (56°C for 30 min) mouse sera, which were pooled from each group, and incubated at room temperature for 25 min, and then transferred to >95% confluent monolayered HeLa cells (ATCC, CCL-2) on a 24-well culture plate.
Techniques: Inhibition, Bacteria, Incubation, Control